Purification of oncolytic measles virus by cation-exchange chromatography using resin-based stationary phases

Cancer therapy with oncolytic measles virus (MV) requires high titers of pure, infectious particles (~108 times more per dose than the vaccine). A gentle but efficient downstream purification process is therefore essential. Here we investigated suitability ion-exchange chromatography for MV using resin-based stationary phases. The success was evaluated based on yield, ratio to total particles, and quantity major contaminants (host cell DNA protein). After testing different anion-exchange, cation-exchange hybrid materials, found that cation-exchanger Eshmuno ® CPX (Merck) most promising resin. By optimizing pH feed buffers, reducing flow rate, achieved a yield 80.7% while by only 16.8%. We also removed 98.3% protein 80.5% host DNA. Although not yet typically used purification, have developed new approach improve efficiency during processing.